protein

Recombinant human RAD1 protein

ATGP0645

10 ug

120 USD

Recombinant human RAD1 protein

1-282aa

ATGP0645

NP_002844.1

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liu Y., et al. (2010) J Biol Chem. 285(8):5974-82.;; Yazinski SA., et al. (2009) Proc Natl Acad Sci u S A. 106(50):21282-7.;;

> 85% by SDS-PAGE

SDS-PAGE

Human

Cell Cycle

33.9 kDa (302aa) confirmed by MALDI-TOF

MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES

His-Tag

E.coli

Recombinant Protein

O60671

RAD1, Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1,ATGP0645-10ug,ATGP0645-20ug,ATGP0645-50ug,ATGP0645-100ug,ATGP0645-250ug,ATGP0645-500ug,ATGP0645-1mg,ATGP0645-10,ATGP0645-20,ATGP0645-50,ATGP0645-100,ATGP0645-250,ATGP0645-500,ATGP0645-1