protein

Recombinant human FKBPL protein

ATGP0623

10 ug

240 USD

Recombinant human FKBPL protein

1-349aa

ATGP0623

NP_071393

FKBPL has similarity to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is thought to have a potential role in the induced radioresistance and it appears to have some involvement in the control of the cell cycle. Also, FKBPL is involved in cellular response to stress. And it is known to interact with Hsp90, glucocorticoid receptor and dynamitin and may play a role in signalling, like other FKBPs. Recombinant FKBPL protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

DIR1, FK506 binding protein like, FKBP prolyl isomerase like, NG7, WAF-1/CIP1 stabilizing protein 39, WISP39

0.25mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

McKeen HD., et al. (2008) Endocrinology 149: 5724.;; Jascur T., et al (2005) Mol. Cell 17 (2): 237-49.;;

> 90% by SDS-PAGE

Specific activity is > 210nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

SDS-PAGE, Enzyme Activity

Human

Heat Shock Proteins

39.2 kDa (357aa) confirmed by MALDI-TOF

Specific activity is > 210 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

METPPVNTIG EKDTSQPQQE WEKNLRENLD SVIQIRQQPR DPPTETLELE VSPDPASQIL EHTQGAEKLV AELEGDSHKS HGSTSQMPEA LQASDLWYCP DGSFVKKIVI RGHGLDKPKL GSCCRVLALG FPFGSGPPEG WTELTMGVGP WREETWGELI EKCLESMCQG EEAELQLPGH SGPPVRLTLA SFTQGRDSWE LETSEKEALA REERARGTEL FRAGNPEGAA RCYGRALRLL LTLPPPGPPE RTVLHANLAA CQLLLGQPQL AAQSCDRVLE REPGHLKALY RRGVAQAALG NLEKATADLK KVLAIDPKNR AAQEELGKVV IQGKNQDAGL AQGLRKMFGL EHHHHHH

His-Tag

E.coli

Recombinant Protein

Q9UIM3

DIR 1, DIR1, DIR-1, FK506 binding protein like, FKBP prolyl isomerase like, FKBPL, NG 7, NG7, NG-7, WAF-1/CIP1 stabilizing protein 39, WISP 39, WISP39, WISP-39, ATGP0623-10ug, ATGP0623-20ug, ATGP0623-50ug, ATGP0623-100ug, ATGP0623-250ug, ATGP0623-500ug, ATGP0623-1mg, ATGP0623-010, ATGP0623-020, ATGP0623-050, ATGP0623-100, ATGP0623-250, ATGP0623-500, ATGP0623-01M