Recombinant human Lactate Dehydrogenase B/LDHB protein | NKMAX ATGP0616 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Lactate Dehydrogenase B/LDHB protein

catalog :

ATGP0616

quantity :

20 ug

price :

70 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Lactate Dehydrogenase B/LDHB protein

Domain :

1-334aa

Catalog number (SKU) :

ATGP0616

NCBI :

NP_002291.1

Description :

LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein, fused to His-tagging at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Read JA., et al. (2001) Proteins. 43(2):175-85. ;; Butt AA., et al. (2002) AIDS. 12(7):317-21. ;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

38.8 kDa (354aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P07195

Internal Search Terms :

Lactate Dehydrogenase B, LDHB,ATGP0616-10ug,ATGP0616-20ug,ATGP0616-50ug,ATGP0616-100ug,ATGP0616-250ug,ATGP0616-500ug,ATGP0616-1mg,ATGP0616-10,ATGP0616-20,ATGP0616-50,ATGP0616-100,ATGP0616-250,ATGP0616-500,ATGP0616-1

more info or order :

NKMAX product webpage