protein

Recombinant human UDP-glucose dehydrogenase/UGDH protein

ATGP0611

10 ug

80 USD

Recombinant human UDP-glucose dehydrogenase/UGDH protein

1-494aa

ATGP0611

NP_003350.1

uGDH is a member of the uDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts uDP-glucose to uDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. Recombinant human uGDH protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1M NaCl, and 1mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bontemps Y., et al. (2003) J Biol Chem. 278(24):21566-75. ;; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70(2):401-10. ;;

> 95% by SDS-PAGE

Specific activity is > 2500pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

59.5 kDa (533aa) confirmed by MALDI-TOF

Specific activity is > 2500 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0pmole of UDP-glucose to UDP-glucuronate per minute at pH 8.7 at 37C.

M FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV

His-Tag

E.coli

Recombinant Protein

O60701

uGDH, uDP-glucose dehydrogenase, GDH, uDP-GlcDH, uDPGDH, uGD, uDP-glucose dehydrogenase, uDP-glucose 6-dehydrogenase,ATGP0611-10ug,ATGP0611-20ug,ATGP0611-50ug,ATGP0611-100ug,ATGP0611-250ug,ATGP0611-500ug,ATGP0611-1mg,ATGP0611-10,ATGP0611-20,ATGP0611-50,ATGP0611-100,ATGP0611-250,ATGP0611-500,ATGP0611-1

Hagiuda D, et al. Clinicopathological and prognostic significance of nuclear UGDH localization in lung adenocarcinoma. (Biomed Res. 2019) {https://pubmed.ncbi.nlm.nih.gov/30787260/}