protein

Recombinant human SUMO E1/SAE1 protein

ATGP0610

10 ug

80 USD

Recombinant human SUMO E1/SAE1 protein

1-346aa

ATGP0610

NP_005491.1

SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

SUMO1 activating enzyme subunit 1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A, activator Of sumo 1, AOS1, SUA1, UBLE1A

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Boggio R., et al. (2007) J Biol Chem. 282(21):15376-82.;; Jones MC., et al. (2006) Proc Natl Acad Sci u S A. 103(44):16272-7.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Ubiquitination

42.2 kDa (378aa) confirmed by MALDI-TOF

MVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK

His-T7-Tag

E.coli

Recombinant Protein

Q9UBE0

SAE1, SuMO1 activating enzyme subunit 1, AOS1, HSPC140, SuA1, uBLE1A, SuMO1 activating enzyme subunit 1, ubiquitin-like 1-activating enzyme E1A, AOS 1, HSPC 140, SuA 1, uBLE 1A, uBL E1A, SAE 1, ubiquitin like protein SuMO 1 activating enzyme,ATGP0610-10ug,ATGP0610-20ug,ATGP0610-50ug,ATGP0610-100ug,ATGP0610-250ug,ATGP0610-500ug,ATGP0610-1mg,ATGP0610-10,ATGP0610-20,ATGP0610-50,ATGP0610-100,ATGP0610-250,ATGP0610-500,ATGP0610-1