protein

Recombinant human PCMT1 protein

ATGP0578

20 ug

70 USD

Recombinant human PCMT1 protein

1-227aa

ATGP0578

AAH08748.1

PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein-L-isoaspartate D-aspartate O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kagan RM., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117(3):379-85.

> 95% by SDS-PAGE

SDS-PAGE

Human

Enzymes & Proteases

28.7 kDa (263aa) confirmed by MALDI-TOF

MAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK

His-Tag

E.coli

Recombinant Protein

P22061

PCMT, Protein-L-isoaspartate O-methyltransferase, Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase, L isoaspartyl/D aspartyl methyltransferase, PCMT 1, PIMT, Protein beta aspartate methyltransferase, Protein L isoaspartate (D aspartate) O methyltransferase, Protein L isoaspartyl/D aspartyl methyltransferase,ATGP0578-10ug,ATGP0578-20ug,ATGP0578-50ug,ATGP0578-100ug,ATGP0578-250ug,ATGP0578-500ug,ATGP0578-1mg,ATGP0578-10,ATGP0578-20,ATGP0578-50,ATGP0578-100,ATGP0578-250,ATGP0578-500,ATGP0578-1

Maeda H, et al. Rapid survey of four Asp isomers in disease-related proteins by LC-MS combined with commercial enzymes. (Anal Chem. 2015){25479244};;Lyon YA, et al. Differences in α-Crystallin isomerization reveal the activity of protein isoaspartyl methyltransferase (PIMT) in the nucleus and cortex of human lenses. (Exp Eye Res. 2018){https://pubmed.ncbi.nlm.nih.gov/29571628/};;Takata T, et al. Site-specific rapid deamidation and isomerization in human lens αA-crystallin in vitro. (Protein Sci. 2020){https://pubmed.ncbi.nlm.nih.gov/31930615/};;Magami K, et al. Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide. (PLoS One. 2021){https://pubmed.ncbi.nlm.nih.gov/33857260/}