protein

Recombinant human FHIT protein

ATGP0563

20 ug

70 USD

Recombinant human FHIT protein

1-147aa

ATGP0563

NP_002003

FHIT (fragile histidine triad) is an enzyme that cleaves adenosine 5' PPP 5' A to yield AMP and ADP. FHIT gene encompasses the common fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. This protein physically associates with ubiquitin conjugating enzyme 9. Recombinant human FHIT, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, AP3Aase, Adenosine 5'-monophosphoramidase FHIT, Adenylylsulfatase, Adenylylsulfate-ammonia adenylyltransferase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FRA3B

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kmiec Z., et al. (2009) J Physiol Pharmacol. 60(4):63-70.;; Ahmad F., et al. (2010) J Cancer Res Clin Oncol. 136(3):333-50.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Enzymes & Proteases

17.9 kDa (155aa) confirmed by MALDI-TOF

MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH

His-Tag

E.coli

Recombinant Protein

P49789

FHIT, Fragile histidine triad protein, Bis (5'-adenosyl)-triphosphatase, AP3Aase, Fragile histidine triad protein AP3A hydrolase, AP3A hydrolase fragile site 3p14.2, Bis 5' adenosyl triphosphatase, Diadenosine 5' 5''' P1 P3 triphosphate hydrolase, Dinucleosidetriphosphatase, FRA 3B, FRA3B, Fragile histidine triad, Fragile histidine triad gene, Fragile histidine triad protein, Tumor suppressor protein.,ATGP0563-10ug,ATGP0563-20ug,ATGP0563-50ug,ATGP0563-100ug,ATGP0563-250ug,ATGP0563-500ug,ATGP0563-1mg,ATGP0563-10,ATGP0563-20,ATGP0563-50,ATGP0563-100,ATGP0563-250,ATGP0563-500,ATGP0563-1

Kawaguchi M, et al. Synthesis of Fluorescent Probes Targeting Tumor-Suppressor Protein FHIT and Identification of Apoptosis-Inducing FHIT Inhibitors. (J Med Chem. 2021){https://pubmed.ncbi.nlm.nih.gov/34160227/}