protein

Recombinant human Glutaredoxin 3/GLRX3 protein

ATGP0551

20 ug

70 USD

Recombinant human Glutaredoxin 3/GLRX3 protein

1-335aa

ATGP0551

NP_006532

Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutaredoxin-3, GRX3, PKC-interacting cousin of thioredoxin, PKC-theta-interacting protein, PKCq-interacting protein, Thioredoxin-like protein 2, Glutaredoxin 4, GLRX4, GRX4, PICOT, TXNL2, HUSSY-22, bA500G10.4

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Witte S., et al. (2000) J Biol Chem 275(3): 1902-9.;; Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394(2):372-6;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Redox Proteins

39.6 kDa (355aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN

His-Tag

E.coli

Recombinant Protein

O76003

TXNL2, TXNL 2, Thioredoxin-like2, Thioredoxin-like protein 2, Thioredoxin-like 2, Thioredoxin like 2, PKC-theta-interacting protein, PKCq-interacting protein, PKCq interacting protein, PKC-interacting cousin of thioredoxin, PKC theta interacting protein, PKC interacting cousin of thioredoxin, PICOT, HUSSY-22, GRX4, GRX3, Glutaredoxin-4, Glutaredoxin4, Glutaredoxin-3, Glutaredoxin3, Glutaredoxin 4, Glutaredoxin 3, GLRX4, GLRX3, bA500G10.4, ATGP0551-10ug, ATGP0551-20ug, ATGP0551-50ug, ATGP0551-100ug, ATGP0551-250ug, ATGP0551-500ug, ATGP0551-1mg, ATGP0551-010, ATGP0551-020, ATGP0551-050, ATGP0551-100, ATGP0551-250, ATGP0551-500, ATGP0551-01M

Kamisuki S, et al. Identification of proteins that bind to the neuroprotective agent neoechinulin A. (Biosci Biotechnol Biochem. 2018){https://pubmed.ncbi.nlm.nih.gov/29447077/}