Recombinant E.coli trxB protein | NKMAX ATGP0540 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli trxB protein

catalog :

ATGP0540

quantity :

20 ug

price :

70 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli trxB protein

Domain :

1-321aa

Catalog number (SKU) :

ATGP0540

NCBI :

YP_003044110.1

Description :

TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Recombinant E. coli TRXB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, FAD/NAD(P)-binding, TRXR

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Faulkner MJ., et al. (2008) Proc Natl Acad Sci u S A. 105(18):6735-40. ;; Takahata M., et al. (2008) J Biochem. 143(4):467-73.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 10unit/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Redox Proteins

Molecular Weight :

34.6 kDa (321aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412nm.

Amino Acid Sequence :

MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P0A9P4

Internal Search Terms :

TRXB, Thioredoxin reductase, Thioredoxin reductase JW0871, ECK0879, Thioredoxin reductase, FAD/NAD(P)-binding, TRXR.,ATGP0540-10ug,ATGP0540-20ug,ATGP0540-50ug,ATGP0540-100ug,ATGP0540-250ug,ATGP0540-500ug,ATGP0540-1mg,ATGP0540-10,ATGP0540-20,ATGP0540-50,ATGP0540-100,ATGP0540-250,ATGP0540-500,ATGP0540-1

more info or order :

NKMAX product webpage