Recombinant human LTA/TNF-beta protein | NKMAX ATGP0517 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human LTA/TNF-beta protein

catalog :

ATGP0517

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human LTA/TNF-beta protein

Domain :

35-205aa

Catalog number (SKU) :

ATGP0517

NCBI :

NP_001153212

Description :

TNF beta, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Recombinant TNF beta protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Messer G, et al. (1991) J Exp Med. 173(1):209-19;; Banner DW, et al. (1993) Cell. 73(3):431-45.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

20.9 kDa (192aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P01374

Internal Search Terms :

Lymphotoxin a1, TNF b, Lymphotoxin alpha, CLTalpha, Coley's toxin, DIF, Differentiation inducing factor, Hemorrhagic factor, LT, LT alpha, LTA, LTalpha, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Natural killer cytotoxic factor, Necrosin, NKCF, OTTHuMP00000037612, OTTHuMP00000037613, TNF B, TNF superfamily member 1, TNFB, TNFbeta, TNFSF 1, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1, Tumor necrosis factor superfamily member 1, Lymphotoxin alpha,ATGP0517-10ug,ATGP0517-20ug,ATGP0517-50ug,ATGP0517-100ug,ATGP0517-250ug,ATGP0517-500ug,ATGP0517-1mg,ATGP0517-10,ATGP0517-20,ATGP0517-50,ATGP0517-100,ATGP0517-250,ATGP0517-500,ATGP0517-1

more info or order :

NKMAX product webpage