Recombinant human Peroxiredoxin 4/PRDX4 protein | NKMAX ATGP0513 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Peroxiredoxin 4/PRDX4 protein

catalog :

ATGP0513

quantity :

20 ug

price :

70 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Peroxiredoxin 4/PRDX4 protein

Domain :

38-271aa

Catalog number (SKU) :

ATGP0513

NCBI :

NP_006397

Description :

PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Alternative Names :

Antioxidant enzyme AOE372, AOE37-2, Peroxiredoxin IV, Prx-IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372, Thioredoxin-dependent peroxiredoxin 4

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jin D.-Y., et al. (1997) J. Biol. Chem. 272:30952-30961.;; Wang HQ., et al. (2009) FEBS Lett. 583(9):1511-5.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >2,000pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

28.8 kDa (255aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.

Amino Acid Sequence :

MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q13162

Internal Search Terms :

Peroxiredoxin4, PRDX4,ATGP0513-10ug,ATGP0513-20ug,ATGP0513-50ug,ATGP0513-100ug,ATGP0513-250ug,ATGP0513-500ug,ATGP0513-1mg,ATGP0513-10,ATGP0513-20,ATGP0513-50,ATGP0513-100,ATGP0513-250,ATGP0513-500,ATGP0513-1

more info or order :

NKMAX product webpage