protein

Recombinant human SOD2/Mn-SOD protein

ATGP0512

20 ug

70 USD

Recombinant human SOD2/Mn-SOD protein

25-222aa

ATGP0512

NP_001019636.1

SOD2 is a member of the iron/manganese superoxide dismutase family. It is a mitochondrial matrix protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this protein have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Recombinant human SOD2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Superoxide dismutase [Mn] mitochondrial, IPOB, MNSOD, MVCD6, Superoxide dismutase [Mn], mitochondrial IPO B, Manganese SOD, Manganese superoxide dismutase, Mn SOD, SOD 2, SOD2, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase 2 mitochondrial, Superoxide Dismutase-2

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

MacMillan-Crow L.A., et al. (1999) Arch. Biochem. Biophys. 366:82-88;; Prunotto M, et al. (2010) J Am Soc Nephrol. 21(3):507-19.;;

> 95% by SDS-PAGE

Specific activity is > 1,000unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

SDS-PAGE, Enzyme Activity

Human

Redox Proteins

24.4 kDa (219aa) confirmed by MALDI-TOF

Specific activity is > 1,000 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.

KHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK

His-Tag

E.coli

Recombinant Protein

P04179

SOD2, Mn-SOD, Superoxide dismutase [Mn] mitochondrial, IPOB, MNSOD, MVCD6, Superoxide dismutase [Mn], mitochondrial IPO B, Manganese SOD, Manganese superoxide dismutase, Mn SOD, SOD 2, SOD2, Superoxide dismutase [Mn] mitochondrial, Superoxide dismutase 2 mitochondrial, Superoxide Dismutase-2,ATGP0512-10ug,ATGP0512-20ug,ATGP0512-50ug,ATGP0512-100ug,ATGP0512-250ug,ATGP0512-500ug,ATGP0512-1mg,ATGP0512-10,ATGP0512-20,ATGP0512-50,ATGP0512-100,ATGP0512-250,ATGP0512-500,ATGP0512-1

Kwon MJ, et al. Superoxide dismutase 3 suppresses hyaluronic acid fragments mediated skin inflammation by inhibition of toll-like receptor 4 signaling pathway: superoxide dismutase 3 inhibits reactive oxygen species-induced trafficking of toll-like receptor 4 to lipid rafts. (Antioxid Redox Signal. 2012) {https://pubmed.ncbi.nlm.nih.gov/21957979/};;Kwon MJ, et al. Superoxide dismutase 3 controls adaptive immune responses and contributes to the inhibition of ovalbumin-induced allergic airway inflammation in mice. (Antioxid Redox Signal. 2012){https://pubmed.ncbi.nlm.nih.gov/22583151/};;Peskin AV, Winterbourn CC. Assay of superoxide dismutase activity in a plate assay using WST-1. (Free Radic Biol Med. 2017){https://pubmed.ncbi.nlm.nih.gov/28017897/}