protein

Recombinant human Dihydrolipoamide Dehydrogenase/DLD protein

ATGP0505

20 ug

70 USD

Recombinant human Dihydrolipoamide Dehydrogenase/DLD protein

36-509aa

ATGP0505

NP_000099

DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the alpha-keto acid dehydrogenase, the pyruvate dehydrogenase, the alpha-ketoglutarate dehydrogenase, the branched-chain alpha-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSuD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Dihydrolipoyl dehydrogenase mitochondrial, DLDH, E3, GCSL, LAD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, DLD, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, Glycine cleavage system L protein, Glycine cleavage system protein L, Lipoamide reductase, Lipoyl dehydrogenase, PHE 3,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Giannopoulou E., et al. (2009) Anticancer Res. 29(12):5077-82. ;; Daee DL., et al. (2009) Proc Natl Acad Sci u S A. 107(1):157-62.;;

> 95% by SDS-PAGE

Specific activity is > 200unit/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.

SDS-PAGE, Enzyme Activity

Human

Metabolism

54.4 kDa (511aa)

Specific activity is > 200 units/mg, one unit will reduce 1.0 umole of DL-lipoamide to DL-dihydrolipoamide per minute at pH 6.5 at 25C.

MADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN ILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD RVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F

His-Tag

E.coli

Recombinant Protein

P09622

Dihydrolipoamide Dehydrogenase, DLD, Dihydrolipoyl dehydrogenase mitochondrial, DLDH, E3, GCSL, LAD, PHE3, Dihydrolipoyl dehydrogenase, mitochondrial Diaphorase, Dihydrolipoamide dehydrogenase, Dihydrolipoyl dehydrogenase, Dihydrolipoyl dehydrogenase mitochondrial, DLD, E3 component of pyruvate dehydrogenase, E3 component of pyruvate dehydrogenase complex 2 oxo glutarate complex branched chain keto acid dehydrogenase complex, Glycine cleavage system L protein, Glycine cleavage system protein L, Lipoamide reductase, Lipoyl dehydrogenase, PHE 3,ATGP0505-10ug,ATGP0505-20ug,ATGP0505-50ug,ATGP0505-100ug,ATGP0505-250ug,ATGP0505-500ug,ATGP0505-1mg,ATGP0505-10,ATGP0505-20,ATGP0505-50,ATGP0505-100,ATGP0505-250,ATGP0505-500,ATGP0505-1