Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

catalog :

ATGP0496

quantity :

20 ug

price :

70 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

Domain :

1-323aa

Catalog number (SKU) :

ATGP0496

NCBI :

NP_001344

Description :

AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228;; Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

38.9 kDa (343aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q04828

Internal Search Terms :

Aldo-keto Reductase1C1, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1, Aldo-keto reductase family 1, member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20 alpha hydroxysteroid dehydrogenase, 2ALPHAHSD, AK1C1, MBAB, Aldo keto reductase family 1 member C1, C9, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRC, DD1, Dihydrodiol dehydrogenase 1, Dihydrodiol dehydrogenase 1/2, H37, HAKRC, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase,ATGP0496-10ug,ATGP0496-20ug,ATGP0496-50ug,ATGP0496-100ug,ATGP0496-250ug,ATGP0496-500ug,ATGP0496-1mg,ATGP0496-10,ATGP0496-20,ATGP0496-50,ATGP0496-100,ATGP0496-250,ATGP0496-500,ATGP0496-1

more info or order :

NKMAX product webpage