protein

Recombinant human Lactate Dehydrogenase A/LDHA protein

ATGP0488

20 ug

70 USD

Recombinant human Lactate Dehydrogenase A/LDHA protein

1-332aa

ATGP0488

NP_005557

LDHA, also known as L-lactate dehydrogenase A chain, is an enzyme that catalyzes the conversion of L-lactate and NAD+ to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. Recombinant LDHA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Lactate dehydrogenase A, L-lactate dehydrogenase A chain, Cell proliferation-inducing gene 19 protein, PIG19, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Gosti F, et al. (1987) Proc. Natl. Acad. Sci. u.S.A. 84(4):1000-4;; Chung FZ, et al. (1985) Biochem. J. 231(3):537-41.;;

> 95% by SDS-PAGE

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Redox Proteins

38.8 kDa (352aa) confirmed by MALDI-TOF

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF

His-Tag

E.coli

Recombinant Protein

P00338

Lactate Dehydrogenase A, LDHA, L-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19, L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein, LDH muscle subunit, M LDH, L lactate dehydrogenase A chain, l7R2, Lactate dehydrogenase 1, A chain, Lactate dehydrogenase A, Lactate dehydrogenase A4, Lactate dehydrogenase M, LDH muscle subunit, OTTMuSP00000017774, Proliferation-inducing gene 19, Renal carcinoma antigen NY-REN-59.,ATGP0488-10ug,ATGP0488-20ug,ATGP0488-50ug,ATGP0488-100ug,ATGP0488-250ug,ATGP0488-500ug,ATGP0488-1mg,ATGP0488-10,ATGP0488-20,ATGP0488-50,ATGP0488-100,ATGP0488-250,ATGP0488-500,ATGP0488-1