protein

Recombinant human Aldolase A/ALDOA protein

ATGP0477

20 ug

70 USD

Recombinant human Aldolase A/ALDOA protein

1-364aa

ATGP0477

NP_908930

Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDA

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Pfleiderer G., et al. (1975) Beitr Pathol. 156(3):266-79. ;; Kishi H., et al. (1987) Proc Natl Acad uSA. 84(23):8623-7.

> 95% by SDS-PAGE

Specific activity is > 10unit/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Metabolism

41.5 kDa (384aa) confirmed by MALDI-TOF

Specific activity is > 10 units/mg, one unit will convert 1.0 umol of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehydes 3-phosphate per minute at pH 7.5 at 37C.

MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY

His-Tag

E.coli

Recombinant Protein

P04075

Aldolase A, Fructose bisphosphate aldolase A, ALDOA, ALDA, GSD12, Fructose bisphosphate aldolase A Aldolase A, Aldolase A fructose bisphosphatase, Aldolase A fructose bisphosphate, Fructose 1 6 bisphosphate triosephosphate lyase, Fructose bisphosphate aldolase, Lung cancer antigen NY Lu 1, MGC10942, MGC17716, MGC17767, Muscle type aldolase,ATGP0477-10ug,ATGP0477-20ug,ATGP0477-50ug,ATGP0477-100ug,ATGP0477-250ug,ATGP0477-500ug,ATGP0477-1mg,ATGP0477-10,ATGP0477-20,ATGP0477-50,ATGP0477-100,ATGP0477-250,ATGP0477-500,ATGP0477-1

Jung SH, et al. Integrative Proteomic Profiling of Protein Activity and Interactions Using Protein Arrays. (Mol Cell Proteomics. 2012){https://www.ncbi.nlm.nih.gov/pubmed/22843993};;Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/}