Recombinant human Aldo-keto reductase 7A3/AKR7A3 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Aldo-keto reductase 7A3/AKR7A3 protein

catalog :

ATGP0476

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Aldo-keto reductase 7A3/AKR7A3 protein

Domain :

1-331aa

Catalog number (SKU) :

ATGP0476

NCBI :

AAH25709.1

Description :

Aldo-keto reductase family 7, member A 3, also known as AKR7A3, is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member includes a number of related monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase, aldose reductase, prostaglandin F synthase, xylose reductase, rho crystallin, and many others. AKR7A3 is involved in the detoxification of aldehydes and ketones. The activity of AKR7A3 may detoxify the aflatoxin B1 (AFB1) dialdehyde, which reacts with proteins, and thereby inhibits AFB 1 induced toxicity. Recombinant human AKR7A3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2(AFAR2), AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kniqht LP., et al. (1999) Carcinogenesis. 20(7):1215-23. ;; Bodreddiqari S., et al. (2008) Chem Res Toxicol. 21(5):1134-42.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

41.6 kDa (370aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Amino Acid Sequence :

M SRQLSRARPA TVLGAMEMGR RMDAPTSAAV TRAFLERGHT EIDTAFVYSE GQSETILGGL GLRLGGSDCR VKIDTKAIPL FGNSLKPDSL RFQLETSLKR LQCPRVDLFY LHMPDHSTPV EETLRACHQL HQEGKFVELG LSNYAAWEVA EICTLCKSNG WILPTVYQGM YNAITRQVET ELFPCLRHFG LRFYAFNPLA GGLLTGKYKY EDKDGKQPVG RFFGNTWAEM YRNRYWKEHH FEGIALVEKA LQAAYGASAP SMTSATLRWM YHHSQLQGAH GDAVILGMSS LEQLEQNLAA AEEGPLEPAV VDAFNQAWHL VAHECPNYFR

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O95154

Internal Search Terms :

Aflatoxin B1 aldehyde reductase member3, AKR7A3, Aldo-keto reductase family 7 member A 3, Aflatoxin B1 aldehyde reductase member 3, AFB1 aldehyde reductase 2(AFAR2), Aldo-keto reductase family 7, member A 3 AFAR 2, AFAR2, AFB1 AR 2, Aflatoxin aldehyde reductase, Aflatoxin B1 aldehyde reductase 2, Aflatoxin B1 aldehyde reductase member 3, Aldo keto reductase family 7 member A3, OTTHuMP00000002623.,ATGP0476-10ug,ATGP0476-20ug,ATGP0476-50ug,ATGP0476-100ug,ATGP0476-250ug,ATGP0476-500ug,ATGP0476-1mg,ATGP0476-10,ATGP0476-20,ATGP0476-50,ATGP0476-100,ATGP0476-250,ATGP0476-500,ATGP0476-1

more info or order :

NKMAX product webpage