protein

Recombinant human HEXIM1 protein

ATGP0475

20 ug

160 USD

Recombinant human HEXIM1 protein

1-359aa

ATGP0475

NP_006451.1

HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant HEXIM1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Hexamethylene bis-acetamide inducible 1, CLP1, EDG1, HIS1, MAQ1, Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1, CLP 1, EDG 1, FLJ13562, Hexamethylene bis acetamide inducible 1, Hexamethylene bis acetamide inducible protein, Hexamethylene bis acetamide inducible transcript 1, HEXIM 1, HEXIM1 protein, HIS 1, HMBA inducible, MAQ 1, Menage a quatre 1.

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Shimizu N., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102(24):8555-60;; Li Q., et al. (2007) Nucleic Acids Res 35(8):2503-12.

> 90% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

44.8 kDa (396aa) confirmed by MALDI-TOF

MAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD

His-Tag

E.coli

Recombinant Protein

O94992

HEXIM1, Hexamethylene bis-acetamide inducible 1, CLP1, EDG1, HIS1, MAQ1, Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1, CLP 1, EDG 1, FLJ13562, Hexamethylene bis acetamide inducible 1, Hexamethylene bis acetamide inducible protein, Hexamethylene bis acetamide inducible transcript 1, HEXIM 1, HEXIM1 protein, HIS 1, HMBA inducible, MAQ 1, Menage a quatre 1.,ATGP0475-10ug,ATGP0475-20ug,ATGP0475-50ug,ATGP0475-100ug,ATGP0475-250ug,ATGP0475-500ug,ATGP0475-1mg,ATGP0475-10,ATGP0475-20,ATGP0475-50,ATGP0475-100,ATGP0475-250,ATGP0475-500,ATGP0475-1