Recombinant human Glutathione S-transferase alpha 1/GSTA1 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glutathione S-transferase alpha 1/GSTA1 protein

catalog :

ATGP0443

quantity :

20 ug

price :

70 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glutathione S-transferase alpha 1/GSTA1 protein

Domain :

1-222aa

Catalog number (SKU) :

ATGP0443

NCBI :

NP_665683

Description :

GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glutathione S transferase A1, Glutathione S-transferase alpha 1, GST epsilon, GST2, GSTA1, GSTA1-1, GTH1, HA subunit 1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Rossi D., et al. (2009) Leukemia. 23(6):1118-26. ;; Hou L., et al. (2007) J Biol Chem. 282(32):23264-74.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 35,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

25.6 kDa (222aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 35,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P08263

Internal Search Terms :

Glutathione S-transferase a1, GSTA1, Glutathione S-transferase alpha 1, GST2, GSTA1-1, GTH1, Glutathione S-transferase alpha 1 Glutathione S transferase A1, GST epsilon, GSTA1, GSTA1 1, HA subunit 1.,ATGP0443-10ug,ATGP0443-20ug,ATGP0443-50ug,ATGP0443-100ug,ATGP0443-250ug,ATGP0443-500ug,ATGP0443-1mg,ATGP0443-10,ATGP0443-20,ATGP0443-50,ATGP0443-100,ATGP0443-250,ATGP0443-500,ATGP0443-1

more info or order :

NKMAX product webpage