Recombinant human FEN1 protein | NKMAX ATGP0442 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human FEN1 protein

catalog :

ATGP0442

quantity :

20 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human FEN1 protein

Domain :

1-380aa

Catalog number (SKU) :

ATGP0442

NCBI :

NP_004102.1

Description :

FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1,

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Hakoyama T., et al. (2009) Nature. 462(7272):514-7. ;; Lal A., et al. (2009) Mol Cell. 35(5):610-25.;;

Purity :

> 90% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

42.5 kDa (380aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P39748

Internal Search Terms :

FEN1, Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1,ATGP0442-10ug,ATGP0442-20ug,ATGP0442-50ug,ATGP0442-100ug,ATGP0442-250ug,ATGP0442-500ug,ATGP0442-1mg,ATGP0442-10,ATGP0442-20,ATGP0442-50,ATGP0442-100,ATGP0442-250,ATGP0442-500,ATGP0442-1

more info or order :

NKMAX product webpage