protein

Recombinant human Ketohexokinase protein

ATGP0437

20 ug

70 USD

Recombinant human Ketohexokinase protein

1-298aa

ATGP0437

AAH06233

Ketohexokinase is an enzyme that catalyzes the phosphorylation of fructose to produce fructose-1-phosphate, leading to consumption of ATP, formation of AMP. This protein initiates first step in the metabolism of dietary fructose and is an important regulator of hepatic glucose metabolism. It is highly found in liver, renal cortex, and small intestine. Its deficiency causes the benign hereditary metabolic disorder essential fructosuria, leading to fructose being excreted in the urine. Recombinant human Ketohexokinase was expressed in E. coli and purified by using conventional chromatography.

KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a,

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bonthron DT., et al. (2009) J Histochem Cytochem. 57(8):763-74.;; Schermerhorn T., et al. (2009) Res Vet Sci. 87(1):115-7. ;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Metabolism

32.7 kDa (298aa) confirmed by MALDI-TOF

MEEKQILCVG LVVLDVISLV DKYPKEDSEI RCLSQRWQRG GNASNSCTIL SLLGAPCAFM GSMAPGHVAD FVLDDLRRYS VDLRYTVFQT TGSVPIATVI INEASGSRTI LYYDRSLPDV SATDFEKVDL TQFKWIHIEG RNASEQVKML QRIDAHNTRQ PPEQKIRVSV EVEKPREELF QLFGYGDVVF VSKDVAKHLG FQSAEEALRG LYGRVRKGAV LVCAWAEEGA DALGPDGKLL HSDAFPPPRV VDTLGAGDTF NASVIFSLSQ GRSVQEALRF GCQVAGKKCG LQGFDGIV

Non-Tagged

E.coli

Recombinant Protein

P50053

Ketohexokinase, KHK, Hepatic fructokinase, EC 2.7.1.3, Hepatic fructokinase, ketohexokinase, Ketohexokinase isoform a,ATGP0437-10ug,ATGP0437-20ug,ATGP0437-50ug,ATGP0437-100ug,ATGP0437-250ug,ATGP0437-500ug,ATGP0437-1mg,ATGP0437-10,ATGP0437-20,ATGP0437-50,ATGP0437-100,ATGP0437-250,ATGP0437-500,ATGP0437-1

Wuest M, et al. Radiopharmacological evaluation of 6-deoxy-6-[18F]fluoro-D-fructose as a radiotracer for PET imaging of GLUT5 in breast cancer. (Nucl Med Biol. 2011){https://pubmed.ncbi.nlm.nih.gov/21531283/}