protein

Recombinant human PKM protein

ATGP0429

20 ug

70 USD

Recombinant human PKM protein

1-531aa

ATGP0429

NP_002645.3

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, is required for bacterial pathogenesis. It is specifically expressed at high levels in tumor cells, and can be measured in plasma of patients with advanced breast cancer. Recombinant human PKM2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Pyruvate kinase muscle isoform M2, Pyruvate kinase M1/2, Pyruvate kinase muscle, Cytosolic thyroid hormone-binding protein, CTHBP, Pyruvate kinase 2/3, Threonine-protein kinase PKM2, Thyroid hormone-binding protein 1, THBP1, Opa-interacting protein 3, OIP3, Tumor M2-PK, Tyrosine-protein kinase PKM2, PK2, PK3, PKM2, p58

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Zwerschke W., et al. (2009). Exp Cell Res. 315(16):2765-74;; Mazurek S., et al. (2007). Ernst Schering Found Symp Proc. (4):99-124.;;

> 95% by SDS-PAGE

Specific activity: > 25,000pmol/min/ug. One unit will convert 1.0pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Cancer

60.1 kDa (551aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P

His-Tag

E.coli

Recombinant Protein

P14618

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