protein

Recombinant human Galectin-3/LGALS3 protein

ATGP0414

20 ug

70 USD

Recombinant human Galectin-3/LGALS3 protein

1-250aa

ATGP0414

NP_002297.2

Galectin-3 is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. Galectin-3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombinant Galectin-3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, MAC2, Advanced glycation end-product receptor 3, GALIG

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Barondes SH., et al. (1994) J Biol Chem. 269(33):20807-10. ;; Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349(1):7-20.

> 95% by SDS-PAGE

The ED50 for this effect is less or equal to 15ug/ml. Measured by its ability to agglutinate human red blood cells.

SDS-PAGE, Bioactivity

Human

Differentiation & Regeneration

28.3 kDa (270aa) confirmed by MALDI-TOF

The ED50 for this effect is less or equal to 15 ug/ml. Measured by its ability to agglutinate human red blood cells.

MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI

His-Tag

E.coli

Recombinant Protein

P17931

Galectin3, CBP35, GAL3, GALBP, GALIG, LGALS2, MAC2., 35 kDa lectin, Carbohydrate binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, Galectin 3 internal gene,included, Galectin-3, Galectin3, GBP, IgE binding protein, IgE-binding protein, L 31, L 34, L-34 galactoside-binding lectin, L31, LGALS3, Laminin-binding protein, Lectin L-29, MAC 2 antigen, Lectin, galactose binding, soluble 3, Mac-2, Macrophage galactose-specific lectin, MGC105387.,ATGP0414-10ug,ATGP0414-20ug,ATGP0414-50ug,ATGP0414-100ug,ATGP0414-250ug,ATGP0414-500ug,ATGP0414-1mg,ATGP0414-10,ATGP0414-20,ATGP0414-50,ATGP0414-100,ATGP0414-250,ATGP0414-500,ATGP0414-1

Campanero-Rhodes, M.A, et al. Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021){https://pubmed.ncbi.nlm.nih.gov/33919637/}