protein

Recombinant mouse Glutathione S-transferase mu 1/GSTM1 protein

ATGP0413

20 ug

70 USD

Recombinant mouse Glutathione S-transferase mu 1/GSTM1 protein

1-218aa

ATGP0413

NP_034488

GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

pmGT10, GSTM1, GST1, GST mu, GST class mu 1, GST class Mu, GST 1-1, Glutathione S-transferase Mu 1, Glutathione S transferase mu

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Goekkurt E., et al. (2009) J Clin Oncol. 27(17):2863-73. ;; Koutros S., et al. (2009) Cancer Res. 69(5):1877-84.

> 95% by SDS-PAGE

Specific activity is > 40,000pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

SDS-PAGE, Enzyme Activity

Mouse

Enzymes & Proteases

28.1 kDa (238aa) confirmed by MALDI-TOF

Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK

His-Tag

E.coli

Recombinant Protein

P10649

pmGT10, GSTM-1, GSTM1, GSTM 1, GST-1, GST1, GST mu, GST class-mu1, GST class-mu 1, GST class mu1, GST class mu 1, GST class mu, GST 1-1, GST 1 1, GST 1, Glutathione S-Transferase Mu1, Glutathione S-transferase Mu 1, Glutathione S Transferase Mu 1, Glutathione S transferase mu, ATGP0413-10ug, ATGP0413-20ug, ATGP0413-50ug, ATGP0413-100ug, ATGP0413-250ug, ATGP0413-500ug, ATGP0413-1mg, ATGP0413-010, ATGP0413-020, ATGP0413-050, ATGP0413-100, ATGP0413-250, ATGP0413-500, ATGP0413-01M