protein

Recombinant human Galectin-4/LGALS4 protein

ATGP0409

20 ug

70 USD

Recombinant human Galectin-4/LGALS4 protein

1-323aa

ATGP0409

NP_006140

Galectin-4 belongs to a subfamily of galectins composed of two carbohydrate recognition domains within the same peptide chain. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions, which inhibits chronic inflammations, GVHD, and allergic reactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. Recombinant Galectin-4 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Gal-4, Antigen NY-CO-27, L-36 lactose-binding protein, L36LBP, Lactose-binding lectin 4

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Huflejt ME., et al. (1997) J Biol Chem. 272(22):14294-303. ;; Huflejt ME., et al. (2004) Glycoconj J. 20(4):247-55.;;

> 95% by SDS-PAGE

The ED50 ≤2ug/ml. Measured by its ability to ability to agglutinate human red blood cells.

SDS-PAGE, Bioactivity

Human

Adhesion & Movement

38.1 kDa (343aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

The ED50 range ≤2 ug/ml. Measured by its ability to ability to agglutinate human red blood cells.

MAYVPAPGYQ PTYNPTLPYY QPIPGGLNVG MSVYIQGVAS EHMKRFFVNF VVGQDPGSDV AFHFNPRFDG WDKVVFNTLQ GGKWGSEERK RSMPFKKGAA FELVFIVLAE HYKVVVNGNP FYEYGHRLPL QMVTHLQVDG DLQLQSINFI GGQPLRPQGP PMMPPYPGPG HCHQQLNSLP TMEGPPTFNP PVPYFGRLQG GLTARRTIII KGYVPPTGKS FAINFKVGSS GDIALHINPR MGNGTVVRNS LLNGSWGSEE KKITHNPFGP GQFFDLSIRC GLDRFKVYAN GQHLFDFAHR LSAFQRVDTL EIQGDVTLSY VQI

His-Tag

E.coli

Recombinant Protein

P56470

Galectin4,ATGP0409-10ug,ATGP0409-20ug,ATGP0409-50ug,ATGP0409-100ug,ATGP0409-250ug,ATGP0409-500ug,ATGP0409-1mg,ATGP0409-10,ATGP0409-20,ATGP0409-50,ATGP0409-100,ATGP0409-250,ATGP0409-500,ATGP0409-1

Campanero-Rhodes, M.A, et al. Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021){https://pubmed.ncbi.nlm.nih.gov/33919637/}