protein

Recombinant S. Japonicum Glutathione S-transferase/GST protein

ATGP0401

20 ug

70 USD

Recombinant S. Japonicum Glutathione S-transferase/GST protein

1-218aa

ATGP0401

AAB59203.1

Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant Schistosoma japonicum GST, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST

1mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hayes JD., et al. (1995). Crit Rev Biochem Mol Biol. 30(6):445-600;; Bekett GJ., et al. (1987). J. Clin Biochem Nutr. 2:1-24;; Smith DB., et al. (1988). Mol Biochem Parasitol. 27(2-3):249-56.;;

> 90% by SDS-PAGE

Specific activity is > 10unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

SDS-PAGE, Enzyme Activity

Schistosoma japonicum

Enzymes & Proteases

28.3 kDa (244aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPK

His-Tag

E.coli

Recombinant Protein

P08515

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