Recombinant human CDC42 protein | NKMAX ATGP0395 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CDC42 protein

catalog :

ATGP0395

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CDC42 protein

Domain :

1-188aa

Catalog number (SKU) :

ATGP0395

NCBI :

NP_001782

Description :

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Cell division cycle 42 isoform 1, G25K, Cell division cycle 42 isoform 1 CDC42Hs, Cell division cycle 42, Small GTP binding protein CDC42.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kathryn P., et al. (2008) J Cell Biol. 183(6):1129-1143. ;; Li R., et al. (1997) J Biol Chem. 272(52):32830-5. ;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

22.4 kDa (203aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Amino Acid Sequence :

MQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC

Tag :

T7-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P60953

Internal Search Terms :

CDC42,ATGP0395-10ug,ATGP0395-20ug,ATGP0395-50ug,ATGP0395-100ug,ATGP0395-250ug,ATGP0395-500ug,ATGP0395-1mg,ATGP0395-10,ATGP0395-20,ATGP0395-50,ATGP0395-100,ATGP0395-250,ATGP0395-500,ATGP0395-1

more info or order :

NKMAX product webpage