Recombinant human Biliverdin Reductase A/BLVRA protein | NKMAX ATGP0377 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Biliverdin Reductase A/BLVRA protein

catalog :

ATGP0377

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Biliverdin Reductase A/BLVRA protein

Domain :

3-296aa

Catalog number (SKU) :

ATGP0377

NCBI :

NP_000703

Description :

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. ( Bilirubin + NAD (P) + = biliverdin + NAD (P) H ) Recombinant BLVRA protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein.

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kravets A., et al. (2004) J Biol Chem. 279(19):19916-23.;; Franklin E., et al. (2007) Biochem J. 405(1):61-7.;;

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

33.3 kDa (295aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of biliverdin of NADPH per minute at pH 8.8 at 25C.

Amino Acid Sequence :

MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P53004

Internal Search Terms :

Biliverdin Reductase A, BLVRA, Biliverdin reductase A, BLVR, BVR, BVRA, BLVRA, Biliverdin reductase A Biliverdin IX alpha reductase, BLVR A, BVR A, Zinc metalloprotein.,ATGP0377-10ug,ATGP0377-20ug,ATGP0377-50ug,ATGP0377-100ug,ATGP0377-250ug,ATGP0377-500ug,ATGP0377-1mg,ATGP0377-10,ATGP0377-20,ATGP0377-50,ATGP0377-100,ATGP0377-250,ATGP0377-500,ATGP0377-1

more info or order :

NKMAX product webpage