Recombinant human Dihydrofolate Reductase/DHFR protein | NKMAX ATGP0371 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Dihydrofolate Reductase/DHFR protein

catalog :

ATGP0371

quantity :

20 ug

price :

55 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Dihydrofolate Reductase/DHFR protein

Domain :

1-187aa

Catalog number (SKU) :

ATGP0371

NCBI :

NP_000782.1

Description :

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Dihydrofolate reductase, EC 1.5.1.3

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Loveridge EJ., et al. (2009) Biochemistry. 48(25):5922-33. ;; Hu Y., et al. (2009) J Cell Biol. 185(1):87-100.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Redox Proteins

Molecular Weight :

23.6 kDa (207aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00374

Internal Search Terms :

Dihydrofolate Reductase, DHFR,ATGP0371-10ug,ATGP0371-20ug,ATGP0371-50ug,ATGP0371-100ug,ATGP0371-250ug,ATGP0371-500ug,ATGP0371-1mg,ATGP0371-10,ATGP0371-20,ATGP0371-50,ATGP0371-100,ATGP0371-250,ATGP0371-500,ATGP0371-1

more info or order :

NKMAX product webpage