protein

Recombinant human DFF45/ICAD protein

ATGP0368

20 ug

80 USD

Recombinant human DFF45/ICAD protein

1-331aa

ATGP0368

AAH07721.1

DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA, also known as DNA fragmentation factor subunit alpha, is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Recombinant DFFA protein was expressed in E. coli and purified by using conventional chromatography techniques.

DNA fragmentation factor subunit alpha., FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHuMP00000001903, OTTHuMP00000001904,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

McCarty JS., et al. (1999) Biochem Biophys Res Commun. 264(1):176-80. ;; Gu J., et al. (1999) J Biol Chem. 274(30):20759-62.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Apoptosis

38.7 kDa (351aa) confirmed by MALDI-TOF

MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL AIDKSLTPVT LVLAEDGTIV DDDDYFLCLP SNTKFVALAS NEKWAYNNSD GGTAWISQES FDVDETDSGA GLKWKNVARQ LKEDLSSIIL LSEEDLQMLV DAPCSDLAQE LRQSCATVQR LQHTLQQVLD QREEVRQSKQ LLQLYLQALE KEGSLLSKQE ESKAAFGEEV DAVDTGISRE TSSDVALASH ILTALREKQA PELSLSSQDL ELVTKEDPKA LAVALNWDIK KTETVQEACE WELALRLQQT QSLHSLRSIS ASKASPPGDL QNPKRARQDP T

His-Tag

E.coli

Recombinant Protein

O00273

DFF45, ICAD, DNA fragmentation factor subunit alpha., FF-45, DFF1, ICAD, DNA fragmentation factor subunit alpha, A330085O09Rik, ICAD L, ICAD S, DFF 1, Caspase activated deoxyribonuclease inhibitor short form, DFF 45, DFF alpha, DFF35, DFF45, DFFA, Caspase-activated deoxyribonuclease inhibitor short form, Dffa DNA fragmentation factor, alpha subunit, DNA fragmentation factor 45 kDa subunit, DNA Fragmentation Factor Alpha Subunit, DNA fragmentation factor subunit alpha, DNA fragmentation factor, 45 kD, alpha subunit, DNA fragmentation factor, 45kDa, alpha polypeptide, DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, RP23 121D17.3, DNA fragmentation factor, alpha subunit, DNAation factor 45 kDa subunit, H13, Inhibitor of CAD, Inhibitor of Caspase Activated DNase, MGC143066, OTTHuMP00000001903, OTTHuMP00000001904,ATGP0368-10ug,ATGP0368-20ug,ATGP0368-50ug,ATGP0368-100ug,ATGP0368-250ug,ATGP0368-500ug,ATGP0368-1mg,ATGP0368-10,ATGP0368-20,ATGP0368-50,ATGP0368-10