protein

Recombinant human Ferredoxin 1/FDX1 protein

ATGP0359

20 ug

80 USD

Recombinant human Ferredoxin 1/FDX1 protein

61-184aa

ATGP0359

NP_004100.1

Ferredoxin1 is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. It participates in the synthesis of thyroid hormones and transfers electrons from adrenodoxin reductase to the cholesterol side chain cleavage cytochrome P450. Recombinant human Ferredoxin 1 protein, fused to T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Adrenodoxin, Adrenal ferredoxin, Hepatoredoxin, ADX, FDX, LOH11CR1D, Adrenodoxin, mitochondrial, FDX1, Ferredoxin 1,

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Mandai T., et al. (2009). FEBS J. 276(8):2416-29. ;; Pichiorri F., et al. (2009). J Biol Chem. 284(2):1040-9.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Redox Proteins

15 kDa (139aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

SSSED KITVHFINRD GETLTTKGKV GDSLLDVVVE NNLDIDGFGA CEGTLACSTC HLIFEDHIYE KLDAITDEEN DMLDLAYGLT DRSRLGCQIC LTKSMDNMTV RVPETVADAR QSIDVGKTS

T7-Tag

E.coli

Recombinant Protein

P10109

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