Recombinant human IL-6 protein | NKMAX ATGP0352 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human IL-6 protein

catalog :

ATGP0352

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human IL-6 protein

Domain :

30-212aa

Catalog number (SKU) :

ATGP0352

NCBI :

NP_000591.1

Description :

Interleukin-6, as known as IL-6, is an immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is also a 'myokine, ' a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 acts as both a pro-inflammatory and anti-inflammatory cytokine. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6. It is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. Recombinant interleukin-6 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternative Names :

Interleukin-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IFNB2, HGF, HSF

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ishihara, et al. (2002) Cytokine Growth Factor Rev. 13(4-5):357-68. ;; Vgontzas AN, et al. (2005) Neuroimmunomodulation.12, 131-40.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.8ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

23.1 kDa (204aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human IL-6 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.8ng/ml.

Amino Acid Sequence :

VPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P05231

Internal Search Terms :

VLAB, VLA-4 subunit bet, VLA4 subunit bet, VLA 4 subunit bet, MSK12, MSK 12, MDF2, Interleukin-6, Interleukin6, Interleukin 6, Interferon beta 2, Integrin beta1, Integrin beta 1, IL-6, IL6, IL 6, IFNB2, IFNB 2, IFN B2, IFN B 2, I L6, HSF, HGF, GPIIA, GP IIA, Glycoprotein Iia, FNRB, Fibronectin receptor subunit beta, CD29, BSF2, BSF 2, ATGP0352-10ug, ATGP0352-20ug, ATGP0352-50ug, ATGP0352-100ug, ATGP0352-250ug, ATGP0352-500ug, ATGP0352-1mg, ATGP0352-010, ATGP0352-020, ATGP0352-050, ATGP0352-100, ATGP0352-250, ATGP0352-500, ATGP0352-01M

more info or order :

NKMAX product webpage