protein

Recombinant human Ataxin-3 protein

ATGP0344

10 ug

80 USD

Recombinant human Ataxin-3 protein

1-370aa

ATGP0344

AAH33711

Ataxin 3 is also known as Machado-Joseph disease protein 1. Machado-Joseph disease is one of several hereditary autosomal dominant neurodegenerative disorders. This protein contains trinucleotide CAG repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. Ataxin 3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB) -binding protein, p300, and p300/CREB-binding protein-associated factor and inhibits transcription by these coactivators. Also, ataxin-3 is a proteasome-associated factor that mediates the degradation of ubiquitinated proteins. Recombinant human Ataxin-3 was expressed in E. coli and purified by using conventional chromatography.

Ataxin 3, ATX3, MJD, MJD1, SCA3, JOS, ATXN3, AT3, EC 3.4.22., Josephin, Machado Joseph disease, Machado Joseph disease protein 1, Machado-Joseph disease protein 1 homolog, MJD gene, Rsca3, SCA3 gene, Spinocerebellar ataxia type 3 protein

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 50mM NaCl, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Doss Pepe EW., et al. (2003). Mol Cell Biol. 23(18):6469-83. ;; Paulson., et al. (1997). Ann Neurol. 41(4):453-62.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Epigenomics (Transcription & Translation)

42.4 kDa (370aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IRVQQMHRPK LIGEELAQLK EQRVHKTDLE RVLEANDGSG MLDEDEEDLQ RALALSRQEI DMEDEEADLR RAIQLSMQGS SRNISQDMTQ TSGTNLTSEE LRKRREAYFE KQQQKQQQQQ QQQQQQQQQQ QQQQGDLSGQ SSHPCERPAT SSGALGSDLG DAMSEEDMLQ AAVTMSLETV RNDLKTEGKK

Non-Tagged

E.coli

Recombinant Protein

P54252

Ataxin3, Ataxin 3, ATX3, MJD, MJD1, SCA3, ATXN3, JOS, ATXN3, AT3, EC 3.4.22., Josephin, Machado Joseph disease, Machado Joseph disease protein 1, Machado-Joseph disease protein 1 homolog, MJD gene, Rsca3, SCA3 gene, Spinocerebellar ataxia type 3 protein,ATGP0344-10ug,ATGP0344-20ug,ATGP0344-50ug,ATGP0344-100ug,ATGP0344-250ug,ATGP0344-500ug,ATGP0344-1mg,ATGP0344-10,ATGP0344-20,ATGP0344-50,ATGP0344-100,ATGP0344-250,ATGP0344-500,ATGP0344-1