protein

Recombinant human Glutathione S-Transferase pi 1/GSTP1 protein

ATGP0341

20 ug

105 USD

Recombinant human Glutathione S-Transferase pi 1/GSTP1 protein

1-210aa

ATGP0341

NP_000843

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

SERPINF1, Serpin peptidase inhibitor clade F member 1, Proliferation inducing protein 35, Pigment epithelium-derived factor EPC 1, Pigment epithelium-derived factor, PIG35, PI, GSTP1, GST3, Glutathione S-Transferase Pi 1, Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III, Glutathione S-transferase P, Glutathione S Transferase Pi, FAEES3, EPC1, DFN7, APF0619

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lee KA, et al. (2001) Blood. 98(12):3483-5.;; Hayes JD, et al. (1995) Crit Rev Biochem Mol Biol. 30(6):445-600.;;

> 95% by SDS-PAGE

Specific activity is > 80unit/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

27.4 kDa (246aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 80 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

MPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ

His-Tag

E.coli

Recombinant Protein

P09211

EPC1, EPC-1, Glutathione S-Transferase pi1, GSTP1, IL 1 A, IL 1 alpha, IL 1A, IL1 alpha, IL-1 alpha, IL1A, IL-1A, IL1alpha, IL1F1, Interleukin 1 alpha, Interleukin 1-alpha, Interleukin-1 alpha, Interleukin-1 alpha precursor, PEDF, PIG 35, PIG35, Pigment epithelium derived factor, Pigment epithelium derived factor EPC 1, Pigment epithelium-derived factor, Pigment epithelium-derived factor EPC 1, Proliferation inducing protein 35, Serpin F1, Serpin peptidase inhibitor clade F member 1, SERPINF 1, SERPINF1, SERPIN-F1, ATGP0341-10ug, ATGP0341-20ug, ATGP0341-50ug, ATGP0341-100ug, ATGP0341-250ug, ATGP0341-500ug, ATGP0341-1mg, ATGP0341-010, ATGP0341-020, ATGP0341-050, ATGP0341-100, ATGP0341-250, ATGP0341-500, ATGP0341-01M