protein

Recombinant human Carbonic Anhydrase 2/CA2 protein

ATGP0325

20 ug

105 USD

Recombinant human Carbonic Anhydrase 2/CA2 protein

1-260aa

ATGP0325

NP_000058.1

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E. coli and purified by using conventional chromatography.

Carbonic anhydrase II, CA-II, Carbonate dehydratase II, Carbonic anhydrase C, CAC, Cyanamide hydratase CA2, Car2

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hu PY., et al. (1992). Hum Mutat. 1(4):288-92 ;; Venta PJ., et al. (1991). Am J Hum Genet. 49(5):1082-90;;

> 95% by SDS-PAGE

Specific activity is > 7,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

29.2 kDa (260aa) confirmed by MALDI-TOF

Specific activity is > 7,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Non-Tagged

E.coli

Recombinant Protein

P00918

Carbonic Anhydrase II, CA2, Carbonic anhydrase 2, CA-II, CAC, CA 2, CA II, CA2, CAI, Car 2, Car2, Carbonate dehydratase II, Carbonic anhydrase B, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase.,ATGP0325-10ug,ATGP0325-20ug,ATGP0325-50ug,ATGP0325-100ug,ATGP0325-250ug,ATGP0325-500ug,ATGP0325-1mg,ATGP0325-10,ATGP0325-20,ATGP0325-50,ATGP0325-100,ATGP0325-250,ATGP0325-500,ATGP0325-1

Saito M, et al. A case of paraneoplastic optic neuropathy and outer retinitis positive for autoantibodies against collapsin response mediator protein-5, recoverin, and α-enolase. (BMC Ophthalmol. 2014){https://pubmed.ncbi.nlm.nih.gov/24428923/}