protein

Recombinant human 4-1BB Ligand/TNFSF9 protein

ATGP0308

20 ug

80 USD

Recombinant human 4-1BB Ligand/TNFSF9 protein

71-254aa

ATGP0308

NP_003802

4-1BB ligand is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with 4-1BBL strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murine models of cancer, infectious disease and autoimmune disease. Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, receptor 4-1BB ligand, homolog of mouse 4-1BB-L, CD137 ligand, CD137L

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lee SW., et al. (2008). Nat Immunol. 9(8):917-26;; Sharma RK., et al. (2009). Cancer Res. 69(10):4319-26;;

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human TNFRSF9 (CAT# ATGP3303).

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

23.8 kDa (222aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human TNFSF9 is coated at 2 ug/ml (100 ul/well) can bind Human TNFRSF9 (CAT# ATGP3303) in a Functional ELISA assay.

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE

His-Tag

E.coli

Recombinant Protein

P41273

41BB Ligand, TNFSF9, Tumor necrosis factor ligand superfamily member 9, TNFSF9, 4-1BBL, CD137L, 4-1BB ligand, 4 1BB L, 4 1BB ligand, 4 1BBL, Cd137l, Cd157l, homolog of mouse 4 1BB L, homolog of mouse 4 1BBL, ILA ligand (TNF related), Ly63l, receptor 4 1BB ligand, Tumor necrosis factor (ligand) superfamily member 9.,ATGP0308-10ug,ATGP0308-20ug,ATGP0308-50ug,ATGP0308-100ug,ATGP0308-250ug,ATGP0308-500ug,ATGP0308-1mg,ATGP0308-10,ATGP0308-20,ATGP0308-50,ATGP0308-100,ATGP0308-250,ATGP0308-500,ATGP0308-1