protein

Recombinant human Smad2 protein

ATGP0307

20 ug

105 USD

Recombinant human Smad2 protein

1-467aa

ATGP0307

NP_005892.1

SMAD2 (Mothers against decapentaplegic homolog 2) belongs to the SMAD family of proteins that mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. Phosphorylated Smad2 is then able to form a complex with Smad4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. Recombinant SMAD2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Sma- and Mad-related protein 2 isoform 1, Sma- and Mad-related protein 2 isoform 1, hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, Sma- and Mad-related protein 2 isoform 1 JV181, MAD, MAD Related Protein 2, SMAD2.

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bonni., et al. (2001) Nat. Cell Biol. 3(6): 587-95.;; Wotton, et al. (1999) Cell. 97(1): 29-39.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Signal Transduction

54.4 kDa (487aa)

< 1 EU per 1ug of protein (determined by LAL method)

MSSILPFTPP VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE LEKAITTQNC NTKCVTIPST CSEIWGLSTP NTIDQWDTTG LYSFSEQTRS LDGRLQVSHR KGLPHVIYCR LWRWPDLHSH HELKAIENCE YAFNLKKDEV CVNPYHYQRV ETPVLPPVLV PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY ELNQRVGETF HASQPSLTVD GFTDPSNSER FCLGLLSNVN RNATVEMTRR HIGRGVRLYY IGGEVFAECL SDSAIFVQSP NCNQRYGWHP ATVCKIPPGC NLKIFNNQEF AALLAQSVNQ GFEAVYQLTR MCTIRMSFVK GWGAEYRRQT VTSTPCWIEL HLNGPLQWLD KVLTQMGSPS VRCSSMS

His-Tag

E.coli

Recombinant Protein

Q15796

Smad2, Sma- and Mad-related protein 2 isoform 1, Sma- and Mad-related protein 2 isoform 1, hMAD-2, hSMAD2, JV18, JV18-1, MADH2, MADR2, Sma- and Mad-related protein 2 isoform 1 JV181, MAD, MAD Related Protein 2, SMAD2.,ATGP0307-10ug,ATGP0307-20ug,ATGP0307-50ug,ATGP0307-100ug,ATGP0307-250ug,ATGP0307-500ug,ATGP0307-1mg,ATGP0307-10,ATGP0307-20,ATGP0307-50,ATGP0307-100,ATGP0307-250,ATGP0307-500,ATGP0307-1

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012){https://pubmed.ncbi.nlm.nih.gov/22843993/};;Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/}