protein

Recombinant human FKBP52/FKBP4 protein

ATGP0303

20 ug

55 USD

Recombinant human FKBP52/FKBP4 protein

1-459aa

ATGP0303

NP_002005.1

FKBP4, also known as FKBP52, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is a component of unactivated mammalian steroid receptor complexes and may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. Recombinant human FKBP4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

FK506 binding protein 4, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, HBI, Hsp 56, HSP binding immunophilin, p52, p59 protein, Peptidylprolyl cis trans isomerase, PPIase, Rotamase, T cell FK506 binding protein 59kD

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Ostrow KL., et al. (2009) Clin Cancer Res. 15(4):1184-91;; Ma D., et al. (2008) J Biol Chem. 283(38):25963-70;;

> 95% by SDS-PAGE

Specific activity is > 700nmol/min/mg, and is defined as the amount of cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

SDS-PAGE, Enzyme Activity

Human

Heat Shock Proteins

53.9 kDa (479aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 700 nmol/min/mg, and is defined as the amount of cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA

His-Tag

E.coli

Recombinant Protein

Q02790

FKBP52, FKBP4, FK506 binding protein 4, FKBP52, FKBP59, HBI, Hsp56, p52, PPIase, FK506 binding protein 4 52 kDa FK506 binding protein, FKBP 4, FKBP 52, FKBP 59, FKBP4, FKBP52 protein, FKBP59, Hsp 56, HSP binding immunophilin, p59 protein, PPIase, Rotamase, Peptidyl prolyl cis trans isomerase, Peptidylprolyl cis trans isomerase, T cell FK506 binding protein 59kD.,ATGP0303-10ug,ATGP0303-20ug,ATGP0303-50ug,ATGP0303-100ug,ATGP0303-250ug,ATGP0303-500ug,ATGP0303-1mg,ATGP0303-10,ATGP0303-20,ATGP0303-50,ATGP0303-100,ATGP0303-250,ATGP0303-500,ATGP0303-1