protein

Recombinant aequorea victoria GFP protein

ATGP0302

20 ug

70 USD

Recombinant aequorea victoria GFP protein

1-238aa

ATGP0302

AAA27721.1

GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.

Green fluorescent protein

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Millan MI, et al. (2009). Medicina. 69(3):370-4.;; Marshall J, et al. (1995). Neuron, 14(2):211-5.;;

> 95% by SDS-PAGE

SDS-PAGE

Aequorea victoria

Fusion Tag & Detection

26.8 kDa (238aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK

Non-Tagged

E.coli

Recombinant Protein

P42212

GFP, Green fluorescent protein,ATGP0302-10ug,ATGP0302-20ug,ATGP0302-50ug,ATGP0302-100ug,ATGP0302-250ug,ATGP0302-500ug,ATGP0302-1mg,ATGP0302-10,ATGP0302-20,ATGP0302-50,ATGP0302-100,ATGP0302-250,ATGP0302-500,ATGP0302-1

Jo J, et al. Cell-penetrating peptide (CPP)-conjugated proteins is an efficient tool for manipulation of human mesenchymal stromal cells. (Sci Rep. 2014){https://www.ncbi.nlm.nih.gov/pubmed/24625570}