protein

Recombinant human Bcl-2 protein

ATGP0297

20 ug

105 USD

Recombinant human Bcl-2 protein

1-211aa

ATGP0297

NP_000624

Bcl-2, also known as B-cell lymphoma protein 2 alpha, is an anti-apoptotic protein located primarily in the outer mitochondrial membrane that blocks the apoptotic death of some cells such as lymphocytes. BCL-2 is thought to regulate cell death by controlling the mitochondrial membrane permeability during apotosis. Bcl-2 exerts its anti-apoptotic function through inhibiting caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). The Bcl-2 gene has been related with a number of cancers, including melanoma, breast, prostate, and lung carcinomas, as well as schizophrenia and autoimmunity. Recombinant Bcl-2 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

BCL2 apoptosis regulator, B-cell CLL/lymphoma 2, PPP1R50, protein phosphatase 1, regulatory subunit 50

0.25mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 2mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Zamzami N., et al. (1998) Oncogene. 16(17):2265-82.;; Fernandez-Sarabia MJ., et al. (1993) Nature. 366(6452):274-5.;;

> 90% by SDS-PAGE

SDS-PAGE

Human

Cancer

25.4 kDa (231aa) confirmed by MALDI-TOF

MGSSHHHHHH SSGLVPRGSH MAHAGRTGYD NREIVMKYIH YKLSQRGYEW DAGDVGAAPP GAAPAPGIFS SQPGHTPHPA ASRDPVARTS PLQTPAAPGA AAGPALSPVP PVVHLTLRQA GDDFSRRYRR DFAEMSSQLH LTPFTARGRF ATVVEELFRD GVNWGRIVAF FEFGGVMCVE SVNREMSPLV DNIALWMTEY LNRHLHTWIQ DNGGWDAFVE LYGPSMRPLF D

His-Tag

E.coli

Recombinant Protein

P10415

Bcl2, B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha, B-cell lymphoma protein 2 alpha Apoptosis regulator Bcl 2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl-2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2.,ATGP0297-10ug,ATGP0297-20ug,ATGP0297-50ug,ATGP0297-100ug,ATGP0297-250ug,ATGP0297-500ug,ATGP0297-1mg,ATGP0297-10,ATGP0297-20,ATGP0297-50,ATGP0297-100,ATGP0297-250,ATGP0297-500,ATGP0297-1

Jung SH, et al. Integrative proteomic profiling of protein activity and interactions using protein arrays. (Mol Cell Proteomics. 2012){https://pubmed.ncbi.nlm.nih.gov/22843993/};;Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/}