Recombinant human VEGF 121/VEGFA protein | NKMAX ATGP0282 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human VEGF 121/VEGFA protein

catalog :

ATGP0282

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human VEGF 121/VEGFA protein

Domain :

207-327aa

Catalog number (SKU) :

ATGP0282

NCBI :

NP_001020541.2

Description :

Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Alternative Names :

Vascular endothelial growth factor A isoform f, Vascular permeability factor, VPF, VEGF

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10 % glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Yasuji ueda., et al. (2004) Cancer Chemother Pharmacol. 54(1):16-24.;; Neufeld G., et al. (1999) FASEB J. 13(1):9-22.;;

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Hormones & Cytokines

Molecular Weight :

14.2 kDa (122aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human VEGF121 stimulates cell proliferation of the HUVEC human umbilical vein endothelial cells. The ED50 range ≤4 ng/ml.

Amino Acid Sequence :

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P15692

Internal Search Terms :

VEGF121,ATGP0282-10ug,ATGP0282-20ug,ATGP0282-50ug,ATGP0282-100ug,ATGP0282-250ug,ATGP0282-500ug,ATGP0282-1mg,ATGP0282-10,ATGP0282-20,ATGP0282-50,ATGP0282-100,ATGP0282-250,ATGP0282-500,ATGP0282-1

more info or order :

NKMAX product webpage