Recombinant E.coli dsbG protein | NKMAX ATGP0281 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli dsbG protein

catalog :

ATGP0281

quantity :

20 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli dsbG protein

Domain :

18-248aa

Catalog number (SKU) :

ATGP0281

NCBI :

NP_415137

Description :

Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E. coli and purified by conventional chromatography techniques.

Alternative Names :

Thiol:disulfide interchange protein dsbG, ybdP, Thiol:disulfide interchange protein dsbG ECK0598, JW0597, Periplasmic disulfide isomerase/thiol disulphide oxidase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Riley M., et al (2006) Nucleic Acids Res. 34(1):1-9.;; Andersen CL., et al (1997) Mol Microbiol. 26(1):121-32;;

Purity :

> 95% by SDS-PAGE

Applications :

SDS-PAGE

Antigen Species :

E.coli

Gene Category :

Heat Shock Proteins

Molecular Weight :

25.8 kDa (232aa) confirmed by MALDI-TOF

Amino Acid Sequence :

MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P77202

Internal Search Terms :

DsbG, Thiol:disulfide interchange protein dsbG, ybdP, Thiol:disulfide interchange protein dsbG ECK0598, JW0597, Periplasmic disulfide isomerase/thiol disulphide oxidase,ATGP0281-10ug,ATGP0281-20ug,ATGP0281-50ug,ATGP0281-100ug,ATGP0281-250ug,ATGP0281-500ug,ATGP0281-1mg,ATGP0281-10,ATGP0281-20,ATGP0281-50,ATGP0281-100,ATGP0281-250,ATGP0281-500,ATGP0281-1

more info or order :

NKMAX product webpage