protein

Recombinant human Dopa Decarboxylase/DDC protein

ATGP0267

20 ug

80 USD

Recombinant human Dopa Decarboxylase/DDC protein

1-480aa

ATGP0267

NP_001076440.2

Dopa decarboxylase, also known as DDC, is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is a protein implicated in 2 metabolic pathways, synthesizing 2 important neurotransmitters: dopamine and serotonin which both play key roles in many clinical disorders, including Parkinson's disease. Dopa decarboxylase is found in different areas of the brain and is particularly abundant in basal ganglia. Recombinant human Dopa decarboxylase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Avgeris M., et al. (2008). Clin Biochem. 41(14-15):1140-9.;; Pons R., et al. (2004). Neruology. 62(7):1058-65.;;

> 95% by SDS-PAGE

SDS-PAGE

Human

Neuroscience

56.4 kDa (503aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

MNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE

His-Tag

E.coli

Recombinant Protein

P20711

Dopa Decarboxylase, DDC, DDC, AADC, Aromatic L-amino acid decarboxylase, L-Dopa decarboxylase, Aromatic L Amino Acid Decarboxylase,ATGP0267-10ug,ATGP0267-20ug,ATGP0267-50ug,ATGP0267-100ug,ATGP0267-250ug,ATGP0267-500ug,ATGP0267-1mg,ATGP0267-10,ATGP0267-20,ATGP0267-50,ATGP0267-100,ATGP0267-250,ATGP0267-500,ATGP0267-1

Uenishi E, et al. A novel mechanism of idiopathic orthostatic hypotension and hypocatecholaminemia due to autoimmunity against aromatic l-Amino acid decarboxylase. (Biochem Biophys Res Commun. 2024){https://pubmed.ncbi.nlm.nih.gov/38677008/}