HT1080 Lysate | NKMAX ATGL0032 product information

product summary

company name :

NKMAX

product type :

other

product name :

HT1080 Lysate

catalog :

ATGL0032

quantity :

100 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Lysates

Item Name :

HT1080 Lysate

Catalog number (SKU) :

ATGL0032

Description :

HT1080 is a fibrosarcoma cell line which has been used extensively in biomedical research. HT-1080 cell line are derived from tissue taken in a biopsy of a fibrosarcoma present in a 35 year-old male. The patient who supplied the sample had not undergone radio or chemotherapy and this lack of therapy is important as both radio and chemotherapy may introduce unwanted mutations into the sample.

Concentration :

2mg/ml (determined by BCA assay)

Formulation :

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage :

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Applications :

WB Comment and Pic :

All lanes : HT1080 Whole Cell Lysate (40ug);; ;; Lane 1. : Anti-GAPDH antibody (ATGA0181);; Lane 2. : Anti-Beta tubulin antibody (ATGA0196);;

Antigen Species :

Human

Gene Category :

Lysates

Preparation before usage :

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

Lysate QC :

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Product Type Name :

Cell Lysate

Internal Search Terms :

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more info or order :

NKMAX product webpage