U-87 MG Lysate | NKMAX ATGL0019 product information

product summary

company name :

NKMAX

product type :

other

product name :

U-87 MG Lysate

catalog :

ATGL0019

quantity :

100 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Lysates

Item Name :

U-87 MG Lysate

Catalog number (SKU) :

ATGL0019

Description :

U-87 MG cells derived from malignant gliomas. This cells are used as an vitro model of human glioblastoma cells to investigate the cytotoxic effect of chemotherapeutic drugs towards cancer cells. The lysate is a good positive control for prostate apoptosis response-4 which is upregulated in prostate tumor cells undergoing apoptosis. It is also a good positive control for Alpha-1-ACT, a serine protease inhibitor tightly associated with amyloid plaques in Alzheimer's disease and in normal aged human and monkey brain.

Concentration :

2mg/ml (determined by BCA assay)

Formulation :

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage :

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Applications :

WB Comment and Pic :

All lanes : U-87 MG Whole Cell Lysate (40ug);; ;; Lane 1. : Anti-GAPDH antibody (ATGA0181);; Lane 2. : Anti-Beta tubulin antibody (ATGA0196);;

Antigen Species :

Human

Gene Category :

Lysates

Preparation before usage :

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

Lysate QC :

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Product Type Name :

Cell Lysate

Internal Search Terms :

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more info or order :

NKMAX product webpage