other

G-361 Lysate

ATGL0012

100 ug

80 USD

G-361 Lysate

ATGL0012

G-361 cells are established from a malignant melanoma of a 31 year-old male. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR) -PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

2mg/ml (determined by BCA assay)

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

WB

All lanes : G-361 Whole Cell Lysate (40ug);; ;; Lane 1. : Anti-GAPDH antibody (ATGA0181);; Lane 2. : Anti-Beta tubulin antibody (ATGA0196);;

Human

Lysates

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Cell Lysate

G361,ATGL0012-10ug,ATGL0012-20ug,ATGL0012-50ug,ATGL0012-100ug,ATGL0012-250ug,ATGL0012-500ug,ATGL0012-1mg,ATGL0012-10,ATGL0012-20,ATGL0012-50,ATGL0012-100,ATGL0012-250,ATGL0012-500,ATGL0012-1