MCF-7 Lysate | NKMAX ATGL0009 product information

product summary

company name :

NKMAX

product type :

other

product name :

MCF-7 Lysate

catalog :

ATGL0009

quantity :

100 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Lysates

Item Name :

MCF-7 Lysate

Catalog number (SKU) :

ATGL0009

Description :

MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.

Concentration :

2mg/ml (determined by BCA assay)

Formulation :

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Storage :

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Applications :

WB Comment and Pic :

All lanes : MCF-7 Whole Cell Lysate (40ug);; ;; Lane 1. : Anti-GAPDH antibody (ATGA0181);; Lane 2. : Anti-Beta tubulin antibody (ATGA0196);;

Antigen Species :

Human

Gene Category :

Lysates

Preparation before usage :

Heat lysate to 95C for 5 minutes and rapidly cooling before use.

Lysate QC :

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Product Type Name :

Cell Lysate

Internal Search Terms :

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more info or order :

NKMAX product webpage