Human IL-15 antibody | NKMAX ATGA0603 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human IL-15 antibody

catalog :

ATGA0603

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT7G9

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human IL-15 antibody

Catalog number (SKU) :

ATGA0603

NCBI :

NP_000576.1

Description :

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays a crucial role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its involvement in a trans-signaling mechanism. In this process, IL-15 receptor alpha (IL-15Rα) from one subset of cells presents IL-15 to neighboring cells that express IL-2Rβ/γc. This cytokine is produced by a wide variety of cells and tissues. Similar to IL-2, IL-15 stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.

Alternative Names :

Interleukin-15, IL15

Concentration :

1mg/ml

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

J G Giri., et al. (1994) EMBO. 13(12):2822-2830.;; Lorenzen I., et al. (2006) J Biol Chem. 281(10):6642-6647.

Abs Host :

Mouse

Isotype :

IgG2b kappa

Immunogen :

Recombinant human IL-15 (49-162aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of IL-15 in HeLa cells. The cell was stained with ATGA0603 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-15 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant human IL-15 protein

Reacts With :

Human

Clone Number :

AT7G9

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of IL-15 in PBMC cells. The cell was stained with ATGA0603 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Immunology, Hormones & Cytokines

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

P40933

Internal Search Terms :

ATGA0603-10ug, ATGA0603-20ug, ATGA0603-50ug, ATGA0603-100ug, ATGA0603-250ug, ATGA0603-500ug, ATGA0603-1mg, ATGA0603-010, ATGA0603-020, ATGA0603-050, ATGA0603-100, ATGA0603-250, ATGA0603-500, ATGA0603-01M

more info or order :

NKMAX product webpage