MERS-CoV Spike RBD antibody | NKMAX ATGA0602 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

MERS-CoV Spike RBD antibody

catalog :

ATGA0602

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AT18D10

application :

western blot, ELISA

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

MERS-CoV Spike RBD antibody

Catalog number (SKU) :

ATGA0602

NCBI :

AFS88936

Description :

MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses. MERS-CoV was first identified in the Kingdom of Saudi Arabia in 2012, which is a single and positive stranded RNA virus. Dromedary camels are widely considered as the source of the transmission of MERS-CoV. The rate of human transmission among household contacts of MERS patients has been approximately 5 % based on serological analysis. MERS-CoV has four structural proteins, known as the S (spike), E (envelope), M (membrane), and N (nucleocapsid) proteins. The spike protein, responsible for allowing the virus to attach to and fuse with the membrane of a host cell and is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. MERS-CoV S mediates viral attachment and fusion to human cells via human cellular receptor DPP4, also known as CD26. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Alternative Names :

Middle East respiratory syndrome coronavirus, Human betacoronavirus 2c EMC/2012, MERS-CoV, MERS, MERS-CoV RBD, MERS RBD, receptor binding domain, RBD, Spike RBD protein

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Junghyun Goo., et al. (2020) Virus Res. 278:197863.;; Yan-Hua Li., et al. (2019) Engineering. 5:940-947.;; Lingshu Wang., et al. (2018) J Virol. 92:e02002-2017.;; Nicolas Papageorgiou., et al. (2016) Acta Crystallogr D Struct Biol. 72:192-202.;; Xiao-Yan Che., et al. (2004) J Clin Microbiol. 42:2629-2635.

Additional Info Comment and Pic :

ELISA: MERS Spike RBD Antibody (1ug/ml) specifically recognizes MERS Spike RBD recombinant protein, but not interacted MERS Spike, Spike S1 and Spike S2 recombinant protein recombinant protein in ELISA.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant MERS-CoV Spike RBD (358-606aa) purified from Baculovirus

Applications :

ELISA, WB

WB Comment and Pic :

The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-MERS Spike RBD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: Recombinant MERS Spike protein (ATGP3979);; Lane 2.: Recombinant MERS Spike S1 protein (ATGP3980);; Lane 3.: Recombinant MERS Spike S2 protein (ATGP3981);; Lane 4.: Recombinant MERS Spike RBD protein (ATGP3982);;

Reacts With :

MERS-CoV

Clone Number :

AT18D10

Ab Usage :

The antibody has been tested by ELISA analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Antigen Species :

MERS-CoV

Gene Category :

Virus

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

K0BRG7

Internal Search Terms :

ATGA0602-10ug, ATGA0602-20ug, ATGA0602-50ug, ATGA0602-100ug, ATGA0602-250ug, covid-19, covid19, sars, sars2,ATGA0602-500ug, ATGA0602-1mg, ATGA0602, ATGA0602-020, ATGA0602-050, ATGA0602-100, ATGA0602-250, ATGA0602-500, ATGA0602-01M

References with this Product :

Lee K, et al. Development of a diagnostic system for detection of specific antibodies and antigens against Middle East respiratory syndrome coronavirus. (Microbiol Immunol. 2018){https://pubmed.ncbi.nlm.nih.gov/30117617/};;Goo J, et al. Characterization of novel monoclonal antibodies against MERS-coronavirus spike protein. (Virus Res. 2020){https://pubmed.ncbi.nlm.nih.gov/31945421/}

more info or order :

NKMAX product webpage