Human PDCD4 antibody | NKMAX ATGA0601 product information

product summary

company name :

NKMAX

product type :

antibody

product name :

Human PDCD4 antibody

catalog :

ATGA0601

quantity :

50 ul

price :

170 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4C2

reactivity :

human

application :

western blot, ELISA, immunocytochemistry, FACS

more info or order :

NKMAX product webpage

product information

Product Type :

Antibodies

Item Name :

Human PDCD4 antibody

Catalog number (SKU) :

ATGA0601

NCBI :

NP_055271

Description :

Programmed cell death 4 (PDCD4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells.

Alternative Names :

Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731

Concentration :

1mg/ml (determined by BCA assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Palamarchuk A, et al., (2006) Cancer Res 65(24):11282-11286.;; Yoshina, et al., (1999) Pathol Int 49(12):1067-1077.

Abs Host :

Mouse

Isotype :

IgG1 kappa

Immunogen :

Recombinant human PDCD4 (1-469aa) purified from E. coli

Applications :

ELISA, WB, ICC/IF, FACS

IF Comment And Pic :

ICC/IF analysis of PDCD4 in HeLa cells. The cell was stained with ATGA0601 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

WB Comment and Pic :

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDCD4 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;; Lane 1.: K562 cell lysate;; Lane 2.: HeLa cell lysate;; Lane 3.: 293T cell lysate

Reacts With :

Human

Clone Number :

4C2

Ab Usage :

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

FACS Comment and Pic :

Flow cytometry analysis of PDCD4 in MCF7 cells. The cell was stained with ATGA0601 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Antigen Species :

Human

Gene Category :

Cancer, Epigenetics and Nuclear Signaling

Conjugation :

Unconjugated

Clonality Mono VS Poly :

Monoclonal

Ab Purification Comment :

By protein-A affinity chromatography

Product Type Name :

Monoclonal antibody

UniProt No. :

Q53EL6

Internal Search Terms :

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more info or order :

NKMAX product webpage